Journal: The American Journal of Pathology

Article Title: The Increased Transforming Growth Factor-β Signaling Induced by Diabetes Protects Retinal Vessels

doi: 10.1016/j.ajpath.2016.11.007

Figure Lengend Snippet: Diabetes and SM16 synergistically alter morphology and density of the retinal capillary network. A: Representative images of retinal trypsin digests, stained using the Lendrum Picro-Mallory method for fibrin, showing normal morphology and density in control rats (C), control rats treated for 3 weeks with SM16 (C+SM16), and diabetic rats with 7 months of diabetes untreated (D), but abnormal vessel morphology, geometry, and density in diabetic rats with 7 months of diabetes treated with SM16 for 3 weeks (D+SM16). All images were taken in midsection of the retinas. B: Bar plot of capillary density (total capillary length per field) in retinal trypsin digests, showing decreased capillary density in D+SM16 rats. Capillary length was measured using the AxioVision software package version 4 (Carl Zeiss) in three areas of midretina, covering 0.1 mm2; in each rat, the lengths measured in the three areas were averaged to yield one value used in the computation. Data are expressed as means ± SD. n = 4 to 5 rats per group. ∗P < 0.05 versus diabetes. Scale bar = 100 μm.

Article Snippet: 15 Capillary density was measured at 20× magnification in three fields in midretina, using the AxioVision software package version 4 (Carl Zeiss, Göttingen, Germany).

Techniques: Staining, Software